Anti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294

Boster Bio Anti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294 (Catalog # M00023-3) . Tested in IHC, WB applications. This antibody reacts with Human.

Product Specifications

Synonyms

Epidermal growth factor receptor;2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB, ERBB1, HER1

Gene Name

EGFR

UniProt

P00533

Host

Rabbit

Reactivity

Human

Cross Reactivity

This antibody reacts to human EGF Receptor (c-ErbB-1) . No cross-reactivity with HER2, HER3, or HER4

Immunogen

A peptide corresponding to the C-terminus of human EGF Receptor (c-ErbB-1) .

Clonality

Monoclonal

Clone

Clone: RM294

Tissue Specificity

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

Applications

IHC, WB

Field of Research

Cancer, Growth Factors/Hormones, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tumor Biomarkers, Tyrosine Kinases

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity) .

Molecular Weight

134277 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Epidermal growth factor receptor

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) .