Anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal Antibody, Clone#RM242

Hypoxia-inducible factor 1-alpha ; HIF-1-alpha ; HIF1-alpha ; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1 ; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1 ; PAS domain-containing protein 8; HIF1A ; BHLHE78, MOP1 , PASD8

Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Apoptosis, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Cell Death, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Host-Virus Interaction, Hypoxia, Interspecies Interaction, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Microbiology, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Response To Hypoxia, Transcription

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity) . Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with HIF3A in the nucleus and speckles (By similarity) . Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (PubMed:9822602) .