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Anti-ZNF147 TRIM25 Antibody

Boster Bio Anti-ZNF147 TRIM25 Antibody (Catalog# A30491) . Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.

Product Specifications

Synonyms

TRIM25; EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147

Gene Name

TRIM25

UniProt

Q14258

Host

Rabbit

Reactivity

Human, Rat, Mouse

Immunogen

A peptide derived from human ZNF147. Immunogen sequence location: 201-250

Clonality

Polyclonal

Tissue Specificity

Expressed in breast tumors (at protein level) . Ubiquitous.

Applications

WB, IHC-P, IF (paraffin), ELISA

Field of Research

Cell Biology, Co-Factors, Epigenetics and Nuclear Signaling, Growth Factors/Hormones, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ring Finger E3 Ligase, Signal Transduction, Transcription, Ubiquitin E3 Enzymes

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784) .

Molecular Weight

70973 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

E3 ubiquitin/ISG15 ligase TRIM25

Subcellular Location

Cytoplasm. Colocalized with DDX58 at cytoplasmic perinuclear bodies.

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