Anti-KLHL22 Monoclonal Antibody

Boster Bio Anti-KLHL22 Monoclonal Antibody catalog # A12497. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Product Specifications

Synonyms

Kelch-like protein 22; KLHL22

Gene Name

KLHL22

UniProt

Q53GT1

Host

Mouse

Reactivity

Human

Immunogen

Purified recombinant fragment of human KLHL22 expressed in E. Coli.

Clonality

Monoclonal

Clone

Clone: 7B1

Applications

WB, IHC, IF

Purification

Ascitic fluid

Form

Liquid

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR (KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. .

Molecular Weight

71667 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Other Gene Names

Kelch-like protein 22

Subcellular Location

Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Mainly. cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase.