Anti-CAND2 Antibody

Anti-CAND2 antibody is ideal for western blotting, ELISA and IHC. CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A) . TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, rat and mouse CAND2/TIP120B. Cross-reactivity does occur with human, rat and mouse CAND1/TIP120A. Cross-reactivity with CAND2 from other sources is not known.

Store CAND2 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

This antibody reacts with human, rat, and mouse CAND2 tested by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from transfected cell lysates (using HeLa and NIH-3T3, and others) . Coimmunoprecipitation of related proteins has not been tested. A 125.4 kDa band corresponding to human CAND2 is detected. CAND2 is specifically expressed in muscle and heart tissue. Researchers should determine optimal titers for other applications.