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Anti-LIM domain-binding protein 2 LDB2 Antibody

Boster Bio Anti-LIM domain-binding protein 2 LDB2 Antibody (Catalog # A09871) . Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Product Specifications

Background

LDB2 (LIM homeobox protein cofactor CLIM-1a) . It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1. LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively.

Synonyms

LDB2, LDB2/CLIM1, CLIM1, LDB-2, CLIM-1, LIM domain-binding protein 2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1, Nuclear LIM interactor

Gene Name

LDB2

UniProt

O55203

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross reactivity with other proteins.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.

Clonality

Polyclonal

Applications

ELISA, IHC, WB

Field of Research

Epigenetics and Nuclear Signaling, Transcription

Purification

This affinity purified antibody is directed against mouse LDB2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross-reactivity with LDB2 homologues from other sources has not been determined.

Concentration

1.06 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Binds to the LIM domain of a wide variety of LIM domain- containing transcription factors.

Molecular Weight

42793 MW

Shipping Conditions

Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=A09871

Product MSDS

https://www.bosterbio.com/msds?sku=A09871

Fragment

IgG

Applications Notes

This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB2 by western blotting in the appropriate cell lysate or extract.

Other Gene Names

LIM domain-binding protein 2

Subcellular Location

Nucleus.

Prediction Reactivity

Chicken, Chimpanzee, Canine

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