Anti-PTEN-P1 PTH2 Antibody

This gene (PTENP1) is a highly homologous pseudogene of PTEN. PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

PTEN-P1, Phosphatase and tensin homolog 2, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein.

This affinity-purified antibody is directed against human PTEN protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against human PTEN protein. A BLAST analysis was used to suggest cross-reactivity with PTEN proteins from mouse, dog sources based on 82% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Plays a role as a potent and selective agonist of PTH2R resulting in adenyl cyclase activation and intracellular calcium levels elevation. Induces protein kinase C beta activation, recruitment of beta-arrestin and PTH2R internalization. May inhibit cell proliferation via its action on PTH2R activation. Neuropeptide which may also have a role in spermatogenesis. May activate nociceptors and nociceptive circuits.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to PTEN-P1 protein by western blotting in the appropriate cell lysate or extract.