Anti-ALG6 Antibody

Anti-ALG6 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA) . Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase is an enzyme that is encoded by the ALG6 gene in humans. This gene encodes ALG6/ALG8 member of glucosyltransferase family. The encoded protein catalyzes the addition of the first glucose residue to the growing lipid-linked oligosaccharide precursor of N-linked glycosylation. Mutations in this gene are associated with congenital disorders of glycosylation type. Anti-ALG6 is ideal for researchers interested in Infectious disease, especially HIV and hepatitis virus infections. Anti-ALG6 antibody is applicable to methods for diagnosis and, in particular, to methods for diagnosing infections using biomarkers targeting exosomes secreted in bodily fluids.

ALG6, ALG-6, Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase, Asparagine-linked glycosylation protein 6 homolog, Dol-P-Glc:Man (9) GlcNAc (2) -PP-Dol alpha-1,3-glucosyltransferase, Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase, ALG6, My046

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of human ALG6 protein, conjugated to KLH.

ALG6 Antibody antiserum is directed against human ALG6. A 58 kDa band is detected when assayed by immunoblot. Cross-reactivity is expected against human ALG6 protein based on a 100% homology of the amino acid sequence of the immunogen between rat, mouse, drosophila, and pongo abelii.

Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man (9) GlcNAc (2) -PP-Dol.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Anti-ALG6 antibody has been tested in ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 58kDa in size corresponding to ALG6 protein by Western blotting in the appropriate cell lysate or extract.