Anti-NF-Y NFYB Antibody

Boster Bio Anti-NF-Y NFYB Antibody (Catalog # A06714-1) . Tested in IHC, WB applications. This antibody reacts with Human.

Product Specifications

Background

Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

Synonyms

NF-Y, Nuclear transcription factor Y subunit beta, Nuclear transcription factor Y subunit B, CAAT box DNA-binding protein subunit B, NF-YB, HAP3

Gene Name

NFYB

UniProt

P25208

Host

Rabbit

Reactivity

Human

Immunogen

NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .

Clonality

Polyclonal

Applications

IHC, WB

Field of Research

Epigenetics and Nuclear Signaling, NFKB Pathway, Nuclear Signaling, Signal Transduction, Signaling Pathway, Transcription

Purification

This product was prepared from monospecific antiserum by delipidation and defibrination.

Concentration

90 mg/mL by Refractometry

Form

Liquid (sterile filtered)

Shipping Conditions

Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Fragment

Antiserum

Applications Notes

This product was tested by immunoblot and found to be reactive against NF-Y (B subunit specific) at a dilution of 1:2,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Minimal reactivity was observed against the A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against NF-Y using 0.5 to 1.0 µl per assay. This product is tested for immunohistochemistry in human thymus tissue at 1:500.