Anti-ELG-1 ATAD5 Antibody

ELG1 (also known as ATP (GTP) -binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.

ELG-1, ELG1, ELG 1, FRAG1, ATP (GTP) -binding protein, ATPase Family AAA Domain-Containing Protein 5, Chromosome fragility associated gene 1 protein, ATAD5, C17orf41

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.

Apoptosis, Cancer, Cell Biology, Cell Cycle, Cell Death, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear, Nucleus, Pathways and Processes

This affinity purified antibody is directed against human Elg1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis of the immunogen suggest full to partial reactivity with several hypothetical proteins. This is not unexpected for such a novel protein. The following proteins should be considered to be highly homologous to Elg1 when using this antibody: human chromosome fragility associated gene 1 (NP_079133), hypothetical dog protein FLJ12735 (XP_548276), hypothetical chimpanzee protein FLJ12735 (XP_511388), and a hypothetical human protein of 1224 residues (CAH10412) . All show 100% homology to the immunogen. Reactivity against homologues from other sources is not known.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Elg1 by western blotting in the appropriate cell lysate or extract.