Anti-CASZ1 Antibody

Boster Bio Anti-CASZ1 Antibody (Catalog # A06443) . Tested in ELISA, WB applications. This antibody reacts with Human.

Product Specifications

Background

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.

Synonyms

CASZ1, CASZ1, Zinc finger protein castor homolog 1, Castor-related protein, Zinc finger protein 693, CST, SRG, ZNF693

Gene Name

CASZ1

UniProt

Q86V15

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross reactivity with other proteins.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, lung, skeletal muscle, pancreas, testis, small intestine, and stomach, but it is not detectable in the adult brain.

Applications

ELISA, WB

Field of Research

Cancer, Domain Families, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, Transcription, Transcription Factors, Tumor Suppressors, Zinc Finger

Purification

This affinity purified antibody is directed against human CASZ1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.

Concentration

1.07 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Transcription factor involved in vascular assembly and morphogenesis through direct transcriptional regulation of EGFL7.

Molecular Weight

190069 MW

Shipping Conditions

Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Fragment

IgG

Applications Notes

This antibody has been tested for use in ELISA, IHC, IF, and western blotting. This antiserum detects endogenous CASZ1 proteins, both the hCASZ5 and hCASZ11 isoforms. Expect a band approximately 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in size corresponding CASZ1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.