Anti-Sipa1 Antibody

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Sipa1 (signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP) . It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. Sipa1 is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1.

Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, G Protein Signaling, Intracellular, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins, Stem Cells, Surface Molecules

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse Sipa1 protein. A BLAST analysis was used to suggest cross-reactivity with Sipa1 from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with Sipa1 from other sources has not been determined.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding