Anti-CAND1 Antibody

CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

CAND1, Cullin associated NEDD8 dissociated protein 1, p120 CAND1, TATA binding protein interacting protein, 20A, TBP interacting protein, TBP interacting protein of 120 kDa A, TIP120

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH.

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, rat and mouse CAND1/TIP120A. Cross-reactivity does occur with human, rat and mouse CAND2/TIP120B. Cross-reactivity with CAND1 from other sources is not known.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

This antibody reacts with human, rat, and mouse CAND1 tested by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa and NIH-3T3, and others) . Coimmunoprecipitation of related proteins has not been tested. A 136.4 kDa band corresponding to human CAND1 is detected. Most cell lines expressing CAND1 can be used as a positive control. Researchers should determine optimal titers for other applications.