Anti-p130 Rb2 RBL2 Antibody

Retinoblastoma-like protein 2 (Rb2) is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. p130 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. It controls histone H4 'Lys-20' trimethylation and probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. It is a potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. It binds to cyclins A and E as well as binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Rb2 may act as a tumor suppressor.

This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 130 kDa Rb2 protein from human, rat and mouse tissue. No reaction was observed against other related tumor suppressor proteins. Cross-reactivity with Rb2 (p130) from other species may also occur.

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Anti-p130 has been tested by western blot and immunohistochemistry and is suitable for ELISA, immunoprecipitation, immunoblotting, immunohistochemistry, and other immunological methods requiring high titer and specificity.