Anti-ARHGEF5 Antibody

Boster Bio Anti-ARHGEF5 Antibody (Catalog # A01487-1) . Tested in WB applications. This antibody reacts with Human, Mouse.

Product Specifications

Background

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.<br><br>Chan A.M.-L., Oncogene 9:1057-1063(1994).<br>Jikuya H., Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.<br>Hillier L.W., Nature 424:157-164(2003)

Synonyms

Rho guanine nucleotide exchange factor 5; Ephexin-3; Guanine nucleotide regulatory protein TIM; Oncogene TIM; Transforming immortalized mammary oncogene; p60 TIM; ARHGEF5; TIM

Gene Name

ARHGEF5

UniProt

Q12774

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from internal of human ARHGEF5.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed with highest levels in placenta. High levels are also found in colon, kidney, trachea, prostate, liver, pancreas, pituitary gland, thyroid gland and mammary gland. In fetal tissues, expressed at high levels in kidney, lung and liver (PubMed:15601624) . Expressed at low levels in lung and heart (PubMed:14662653) . .

Applications

Field of Research

G Protein Signaling, Signal Transduction, Signaling Pathway, Small G Proteins

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Dilution

Western blotting: 1:500~1:3000

Form

Liquid

Function

Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624) . Strongly activates RHOA (PubMed:15601624) . Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity) . Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624) . Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653) . Required for SRC-induced podosome formation (By similarity) . Involved in positive regulation of immature dendritic cell migration (By similarity) . .