Anti-HDAC-5 (internal) Antibody

HDAC5 is a ubiquitous protein responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. A tag for epigenetic repression, Anti-HDAC5 is an enzyme involved in transcriptional regulation, cell cycle progression and developmental events. HDAC5 is a class II histone deacetylase as defined by its similarities to other members of this class. HDAC5 is a major player in muscle differentiation, acting by repressing transcription of myocyte enhancer MEF2C. During differentiation it moves from the nucleus into the cytoplasm, allowing for myocyte enhancer factor expression. Anti-HDAC5 antibody is ideal for researchers interested in Stem Cell, Cardiovascular, or Epigenetics and Nuclear Signaling research.

Anti-HDAC5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 325-375aa of human HDAC5 protein.

Anti-HDAC5 was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with human and hamster based on 100% sequence homology. Cross-reactivity with HDAC5 from other sources has not been determined.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Anti-HDAC5 antibody tested in ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~122kDa corresponding to the appropriate cell lysate or extract.