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Biotinylated HCoV-229E Spike Trimer Protein (TI 871-872 PP), His, Avitag™ (MALS verified)

Human coronavirus 229E (HCoV-229E) is one of seven known coronaviruses to infect humans. Likely originated from bats, it is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to the APN receptor. Along with Human coronavirus OC43 (a member of the Betacoronavirus genus), it is one of the viruses responsible for the common cold. The spike protein of HCoV-229E is a trimer with subunits S1 and S2 responsible for host receptor binding and fusion of the viral and host cell membranes, respectively.

Product Specifications

Background

Human coronavirus 229E (HCoV-229E) is one of seven known coronaviruses to infect humans. Likely originated from bats, it is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to the APN receptor. Along with Human coronavirus OC43 (a member of the Betacoronavirus genus), it is one of the viruses responsible for the common cold. The spike protein of HCoV-229E is a trimer with subunits S1 and S2 responsible for host receptor binding and fusion of the viral and host cell membranes, respectively.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 128.4 kDa. The protein migrates as 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Accession Number

P15423 (TI 871-872 PP)

Expression Region

Cys 16 - Pro 1115

Host

HEK293

Target

Spike protein

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

HCoV-229E

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Additives

Trehalose

Molecular Weight

128.4 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Product Datasheet

https://console.acrobiosystems.com/public/product/pdf/ds/SPN-H82E8.pdf

Host or Source

HEK293

Species

HCoV-229E

Protein ID

P15423 (TI 871-872 PP)

Preservative

Trehalose

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