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Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin

Streptavidin is expressed from E. coli cells and conjugated with horseradish peroxidase under optimal conditions.

Product Specifications

Background

Streptavidin is a 66 kDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44 kDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.

Specifications

This protein carries no "tag". The protein has a calculated MW of 13.8 kDa.

Host

E. coli

Target

Streptavidin

Conjugation

HRP Labeled

Tag

Native

Source

Streptomyces avidinii

Applications

NOTE: Do not use skim milk as a blocking agent in the assay with streptavidin, since skim milk contains free biotin which will cause high backgrounds.

Stability

-20°C to -70°C for 2 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Bioactivity

Immobilized Cynomolgus / Rhesus macaque BCMA, His Tag (Cat. No. BCA-C52H7) at 1 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 0.5-16 ng/mL when detected by Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (0.1 µg/mL) (Cat. No. STN-NH913) (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

13.8 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C, avoid light

Package Size

100ug*1

Product Datasheet

https://console.acrobiosystems.com/public/product/pdf/ds/STN-NH913.pdf

Host or Source

E. coli

Species

Streptomyces avidinii

Preservative

Trehalose

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