Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin
Streptavidin is expressed from E. coli cells and conjugated with horseradish peroxidase under optimal conditions.
Product Specifications
Background
Streptavidin is a 66 kDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44 kDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.
Specifications
This protein carries no "tag". The protein has a calculated MW of 13.8 kDa.
Host
E. coli
Target
Streptavidin
Conjugation
HRP Labeled
Tag
Native
Source
Streptomyces avidinii
Applications
NOTE: Do not use skim milk as a blocking agent in the assay with streptavidin, since skim milk contains free biotin which will cause high backgrounds.
Stability
-20°C to -70°C for 2 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Bioactivity
Immobilized Cynomolgus / Rhesus macaque BCMA, His Tag (Cat. No. BCA-C52H7) at 1 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 0.5-16 ng/mL when detected by Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (0.1 µg/mL) (Cat. No. STN-NH913) (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
13.8 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C, avoid light
Package Size
100ug*1
Host or Source
E. coli
Species
Streptomyces avidinii
Preservative
Trehalose
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