Recombinant Canine distemper virus Nucleoprotein (N), partial

Product Specifications

Product Name Alternative

Nucleocapsid protein

Abbreviation

Recombinant Canine distemper virus N protein, partial

Gene Name

UniProt

P04865

Expression Region

399-523aa

Organism

Canine distemper virus (strain Onderstepoort) (CDV)

Target Sequence

SKTTEDRTIRATGPKQSQITFLHSERSEVANQQPPTINKRSENQGGDKYPIHFSDERLPGYTPDVNSSEWSESRYDTQIIQDDGNDDDRKSMEAIAKMRMLTKMLSQPGTSEDNSPVYSDKELLN

Tag

C-terminal 6xHis-Myc-tagged

Type

Developed Protein

Source

Yeast

Field of Research

Microbiology

Relevance

Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity) .

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

17.9 kDa

References & Citations

"Canine distemper terminal and intergenic non-protein coding nucleotide sequences: completion of the entire CDV genome sequence." Sidhu M.S., Husar W., Cook S.D., Dowling P.C., Udem S.A. Virology 193:66-72 (1993)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.