Anti-FPR 1/2 Antibody

Mouse Monoclonal Antibody specific to FPR1/2 MAb

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

fMLP receptor, N-formyl peptide receptor, FPR, N-formylpeptide chemoattractant receptor

Gene Name

FPR1

NCBI Gene ID

UniProt

P21462

Cellular Locus

Cell membrane

Host

Mouse

Reactivity

Human

Immunogen

His-tagged recombinant FPR1 (human neutrophil N-formyl peptide receptor 1) expressed in and purified from Sf9 cells.

Target Antigen

fMet-Leu-Phe receptor

Target

FPR 1/2

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IF

Field of Research

Growth Factors, Cytokines, Receptors

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

High affinity receptor for N-formyl-methionyl peptides (fMLP), which are powerful neutrophil chemotactic factors (PubMed:2161213, PubMed:2176894, PubMed:10514456, PubMed:15153520). Binding of fMLP to the receptor stimulates intracellular calcium mobilization and superoxide anion release (PubMed:2161213, PubMed:1712023, PubMed:15153520, PubMed:15210802). This response is mediated via a G-protein that activates a phosphatidylinositol-calcium sPubMed:10514456, PubMed:15153520, PubMed:2161213, PubMed:2176894, PubMed:25109685, PubMed:10514456, PubMed:1712023, PubMed:2161213, PubMed:2176894}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1)year at -20°C to -70 C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human FPR1 at aa 305-GQDFRERLI-313 at the amino- terminal portion of the carboxyl-terminal tail. It also recognizes the same epitope in FPR2.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Formyl peptide receptors (FPR) are G protein-coupled receptors that are activated by different agonists included N- formylated peptides originating from bacteria and mitochondria. In humans, the FPR family includes the high affinity FPR1, the low affinity FPR2/ALX (previously known as FPRL1 or ALX) and FPR3. Human FPR1 is important for phagocyte chemotaxis, superoxide production, and degranulation, and helps direct phagocytes to sites of infection. Functional FPR1expression has been demonstrated on fibroblasts, bone marrow mesenchymal stem cells, A549 lung cells, HEP-G2 hepatoma cells, and on several types of epithelial cells.