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MC5R Antibody

MC5R Antibody

Product Specifications

Product Name Alternative

MC5R N: melanocortin 5 receptor

UniProt

P33032

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: PNVKNKSSPCED

Target

MC5R

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 37kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 36.6kDa according to NP_005904.1) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptideImmunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 2.5μg/mL.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_005904.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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