Mcpt4 Antibody
Mcpt4 Antibody
Product Specifications
Product Name Alternative
Mcpt4, mast cell protease 4, MMCP-4, MMCP-4A, MMCP-4B, Mcp-4, myonase
UniProt
P21812
Reactivity
Mouse
Immunogen
The immunogen for this antibody is: C-KLQKKAKETPSVN
Target
Mcpt4
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx. 40kDa and 15kDa in Mouse Skeletal Muscle lysates after 0.01μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 27.2kDa according to NP_034909.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:Paraffin embedded Mouse Skeletal Muscle. Recommended concentration: 3.75μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_034909.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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