What is the catalog number for NEFM Antibody?

The catalog number for NEFM Antibody is orb1249453.

What sizes are available for NEFM Antibody?

NEFM Antibody is available in the following size(s): 0.1 mg.

Is NEFM Antibody in stock?

NEFM Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

NEFM Antibody

Product Specifications

Product Name Alternative

NEFM, neurofilament, medium polypeptide, NEF3, NF-M, NFM, neurofilament triplet M protein, neurofilament, medium polypeptide 150kDa, neurofilament-3 (150 kD medium)

UniProt

P07197

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-QASHITVERKDYLK

Target

NEFM

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 5μg/mL.

Prediction Reactivity

Bovine, Canine, Porcine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_005373.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.