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CEP290 Antibody

CEP290 Antibody

Product Specifications

Product Name Alternative

CEP290, centrosomal protein 290kDa, 3H11Ag, BBS14, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, NPHP6, SLSN6, rd16, CTCL tumor antigen se2-2, monoclonal antibody 3H11 antigen, nephrocytsin-6, prostate cancer antigen T21

UniProt

O15078

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RNSKHLKQQQYRAEN

Target

CEP290

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Kidney, Testis, and HeLa lysates after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 290kDa according to NP_079390.3. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunocytochemistry: Formaldehyde-fixed cell lines derived from Prostate Cancer and from Breast Cancer show typical centrosomal staining. Recommended concentration: 2-3μg/mL

Tested Applications

ELISA, ICC

NCBI Accession Number

NP_079390.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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