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BOK Antibody

BOK Antibody

Product Specifications

Product Name Alternative

BOK, BCL2-related ovarian killer, BCL2L9, BOKL, MGC4631

UniProt

Q9UMX3

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-HISLQSEPVVTD

Target

BOK

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 20kDa in Human Testis lysates after 1μg/mL antibody staining, and 17kDa in Mouse Spleen and Thymus lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 23.3kDa according to NP_115904.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.

Prediction Reactivity

Bovine, Mouse

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_115904.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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