Tph2 Antibody
Tph2 Antibody
Product Specifications
Product Name Alternative
Tph2, tryptophan hydroxylase 2, AU043594, MGC159133, Ntph, OTTMUSP00000022885, neuronal tryptophan hydroxylase, tryptophan 5-hydroxylase 2, tryptophan 5-monooxygenase 2
UniProt
Q8CGV2
Reactivity
Mouse
Immunogen
The immunogen for this antibody is: C-SLTQNKAIKSEDK
Target
Tph2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 85kDa band in Mouse fetal Brain lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 55.9kDa according to NP_775567.2. The 85kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Mouse Brain stem shows staining of Raphe nucleii serotonergic neurons consistent with observation by different antibodies. Recommended concentration, 0.5-2μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_775567.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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