What is the catalog number for Sirt3 Antibody?

The catalog number for Sirt3 Antibody is orb1249278.

What sizes are available for Sirt3 Antibody?

Sirt3 Antibody is available in the following size(s): 0.1 mg.

Is Sirt3 Antibody in stock?

Sirt3 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

Sirt3 Antibody

Product Specifications

Product Name Alternative

Sirt3, sirtuin 3, sirtuin 3 (silent mating type information regulation 2, homolog) 3, 2310003L23Rik, AI848213, Sir2l3, NAD-dependent deacetylase sirtuin-3, OTTMUSP00000027441, OTTMUSP00000027442, OTTMUSP00000027444, OTTMUSP00000027445, SIR2-like protein 3, SIRT3L mitochondrial, mSIR2L3, mitochondrial protein lysine deacetylase, silent mating type information regulation 2, (S.cerevisiae, homolog) -like 3

UniProt

Q9NTG7

Reactivity

Mouse

Immunogen

The immunogen for this antibody is: C-RASGIPASKLVEAH

Target

Sirt3

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Mouse Heart lysates (calculated MW of 28.8kDa according to NP_071878.2) . An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Kidney for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Human, Porcine, Rat

Tested Applications

ELISA, FC, IF, WB

NCBI Accession Number

NP_071878.2, NP_001171275.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.