PRDM14 Antibody

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 40kDa band in Human Heart lysates after 0.1-1μg/mL antibody staining and this band was successfully blocked by incubation with the immunizing peptide. Preliminary experiments also gave an approxand an approx 48-50kDa band in Human Lung and Kidney lysates and in nuclear lysates of cell liines Jurkat, HEK293 and MCF7. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 64.1kDa according to Human NP_078780.1.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.