CASP3 Antibody
CASP3 Antibody
Product Specifications
Product Name Alternative
CASP3, caspase 3, caspase 3, apoptosis-related cysteine protease, HGNC:1504, caspase 3, apoptosis-related cysteine peptidase, CPP32, CPP32B, SCA-1, PARP cleavage protease, SREBP cleavage activity 1, Yama, apopain, cysteine protease CPP32, procaspase3
UniProt
P42574
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-RDVSKEDHSKRS
Target
CASP3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 32kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6kDa according to Human NP_004337.2 and 31.4kDa according to Mouse NP_033940.1, NP_001271338.1) . An additional band of 65kDa was also consistently observed in MOLT4 and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Canine, Rat
Tested Applications
ELISA, IF, WB
NCBI Accession Number
NP_116786.1, NP_004337.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
