LIG1 Antibody

Product Specifications

Product Name Alternative

LIG1, ligase I, DNA, ATP-dependent, MGC117397, MGC130025, DNA ligase I

UniProt

P18858

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RVREDKQPEQATTS

Target

LIG1

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3μg/mL.

Prediction Reactivity

Rat

Tested Applications

ELISA, WB

NCBI Accession Number

NP_000225.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.