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Cytokeratin 20 Antibody

Cytokeratin 20 Antibody

Product Specifications

Product Name Alternative

KRT20, keratin 20, CK-20, CK20, K20, KRT21, MGC35423, OTTHUMP00000164518, cytokeratin 20, cytokeratin-20, keratin, type I cytoskeletal 20, keratin-20, protein IT

UniProt

P35900

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-NSRHTVNYGSDLT

Target

Cytokeratin 20

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Molecular Weight

Approx 49kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 49kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5kDa according to NP_061883.1) . Recommended concentration: 0.01-0.03μg/mL.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA as a capture antibody in combiation with reporter antibody EB11745.

Tested Applications

EIA, ELISA, WB

NCBI Accession Number

NP_061883.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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