Myosin IH Antibody
Myosin IH Antibody
Product Specifications
Product Name Alternative
MYO1H, myosin IH, myosin-1H, myosin-Ih, unconventional myosin-Ih
UniProt
Q8N1T3
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-EDQVYKDKNGQLR
Target
Myosin IH
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Molecular Weight
Approx 110kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 118kDa according to NP_001158045.1) . Recommended concentration: 0.1-0.3μg/mL. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.
Prediction Reactivity
Bovine, Porcine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001158045.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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