GDF15 Antibody
GDF15 Antibody
Product Specifications
Product Name Alternative
GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, growth differentiation factor 15, PTGF-beta, prostate differentiation factor, NSAID (nonsteroidal anti-inflammatory drug) -activated protein 1
UniProt
Q99988
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QKTDTGVSLQTYDD
Target
GDF15
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 30-35 kDa band observed in Human Testis lysates (calculated MW of 34.1kDa according to NP_004855.2) . Recommended concentration: 0.1-0.3μg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Skin lysate. In transfected HEK293 transiently expressing GDF15 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293. Preliminary testing was unsuccessful on Mouse for this particular batch. IHC: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 5μg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, FC, IHC, WB
NCBI Accession Number
NP_004855.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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