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GDF15 Antibody

GDF15 Antibody

Product Specifications

Product Name Alternative

GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, growth differentiation factor 15, PTGF-beta, prostate differentiation factor, NSAID (nonsteroidal anti-inflammatory drug) -activated protein 1

UniProt

Q99988

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-QKTDTGVSLQTYDD

Target

GDF15

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 30-35 kDa band observed in Human Testis lysates (calculated MW of 34.1kDa according to NP_004855.2) . Recommended concentration: 0.1-0.3μg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Skin lysate. In transfected HEK293 transiently expressing GDF15 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293. Preliminary testing was unsuccessful on Mouse for this particular batch. IHC: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 5μg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, FC, IHC, WB

NCBI Accession Number

NP_004855.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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