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RBP1 Antibody

RBP1 Antibody

Product Specifications

Product Name Alternative

RBP1, CRBP, RBPC, CRBP1, CRABP-I, retinol binding protein 1, cellular, retinol-binding protein 1, cellular, CRBPI

UniProt

P09455

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: VEGVVCKQVFKKVQ

Target

RBP1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 16kDa band observed in NIH3T3 and approx 17kDa in U251 cell lysates. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. (calculated MW of 22.3kDa according to Human NP_002890.2 and 15.8kDa according to Mouse NP_035384.1) . Recommended concentration: 0.5-3μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine

Tested Applications

ELISA, FC, IF, WB

NCBI Accession Number

NP_002890.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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