SNAP25 Antibody
SNAP25 Antibody
Product Specifications
Product Name Alternative
SNAP25, synaptosomal-associated protein, 25kDa , HGNC:11132, FLJ23079, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2 , synaptosomal-associated protein 25, RIC-4, RIC4, SEC9, resistance to inhibitors of cholinesterase 4 homolog, synaptosomal-associated protein 25
UniProt
P60880
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-DEANQRATKMLGSG
Target
SNAP25
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Cerebellum and in Mouse and Rat Brain lysates (calculated MW of 23.3kDa according to Human NP_003072.2, Mouse NP_035558.1 and Rat NP_001257504.1) . Recommended concentration: 0.03-0.1μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong axon staining, particularly in the granular region. Recommended concentration: 1-2μg/mL.
Prediction Reactivity
Canine
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_003072.2, NP_570824.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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