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SNAP25 Antibody

SNAP25 Antibody

Product Specifications

Product Name Alternative

SNAP25, synaptosomal-associated protein, 25kDa , HGNC:11132, FLJ23079, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2 , synaptosomal-associated protein 25, RIC-4, RIC4, SEC9, resistance to inhibitors of cholinesterase 4 homolog, synaptosomal-associated protein 25

UniProt

P60880

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-DEANQRATKMLGSG

Target

SNAP25

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Cerebellum and in Mouse and Rat Brain lysates (calculated MW of 23.3kDa according to Human NP_003072.2, Mouse NP_035558.1 and Rat NP_001257504.1) . Recommended concentration: 0.03-0.1μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong axon staining, particularly in the granular region. Recommended concentration: 1-2μg/mL.

Prediction Reactivity

Canine

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_003072.2, NP_570824.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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