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TSC1 Antibody

TSC1 Antibody

Product Specifications

Product Name Alternative

TSC1, hamartin, tuberous sclerosis 1 , HGNC:12362, KIAA0243, LAM, MGC86987, TSC, tuberous sclerosis 1 protein, tumor suppressor

UniProt

Q92574

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-QLHIMDYNETHHEH

Target

TSC1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9 (12) :1721-7; PMID: 10915759) . In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3μg/mL.Immunohistochemistry:Paraffin embedded Human Uterus and Liver. Recommended concentration: 3.75μg/ml.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_000359.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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