TSC1 Antibody
TSC1 Antibody
Product Specifications
Product Name Alternative
TSC1, hamartin, tuberous sclerosis 1 , HGNC:12362, KIAA0243, LAM, MGC86987, TSC, tuberous sclerosis 1 protein, tumor suppressor
UniProt
Q92574
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QLHIMDYNETHHEH
Target
TSC1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9 (12) :1721-7; PMID: 10915759) . In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3μg/mL.Immunohistochemistry:Paraffin embedded Human Uterus and Liver. Recommended concentration: 3.75μg/ml.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_000359.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog