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FARP1 Antibody

FARP1 Antibody

Product Specifications

Product Name Alternative

FARP1, FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived), CDEP, chondrocyte-derived ezrin-like protein, RP11-111L24.1, MGC87400, PLEKH2, FERM, RhoGEF, and pleckstrin domain protein 1

UniProt

Q9Y4F1

Reactivity

Human

Immunogen

The immunogen for this antibody is: GEIEQRPTPGSRL-C

Target

FARP1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments showed an approx 110-120kDa band in Human Frontal Cortex lysates (calculated MW of 119kDa according to NP_ 005757.1) .. An additional band was also consistently observed at 40kDa and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Mouse, Rat

Tested Applications

ELISA, FC, IF

NCBI Accession Number

NP_001001715.2, NP_005757.1, NP_001273768.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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