DDAH1 Antibody

Product Specifications

Product Name Alternative

DDAH1, dimethylarginine dimethylaminohydrolase 1, DDAH, DDAHI, NG, NG-dimethylarginine dimethylaminohydrolase, RP4-621F18.1, FLJ21264, FLJ25539

UniProt

O94760

Reactivity

Human

Immunogen

The immunogen for this antibody is: TCCSVLINKKVDS

Target

DDAH1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 35kDa band observed in Human Kidney, Human Brain (Cerebellum) and Pancreas lysates (calculated MW of 31.1kDa according to NP_036269.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J. 2005 Jul;19 (9) :1175-7; PMID: 15827267) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.

Prediction Reactivity

Bovine

Tested Applications

ELISA, WB

NCBI Accession Number

NP_036269.1, NP_001127917.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.