DDAH1 Antibody
DDAH1 Antibody
Product Specifications
Product Name Alternative
DDAH1, dimethylarginine dimethylaminohydrolase 1, DDAH, DDAHI, NG, NG-dimethylarginine dimethylaminohydrolase, RP4-621F18.1, FLJ21264, FLJ25539
UniProt
O94760
Reactivity
Human
Immunogen
The immunogen for this antibody is: TCCSVLINKKVDS
Target
DDAH1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 35kDa band observed in Human Kidney, Human Brain (Cerebellum) and Pancreas lysates (calculated MW of 31.1kDa according to NP_036269.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J. 2005 Jul;19 (9) :1175-7; PMID: 15827267) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.
Prediction Reactivity
Bovine
Tested Applications
ELISA, WB
NCBI Accession Number
NP_036269.1, NP_001127917.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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