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ELF1 Antibody

ELF1 Antibody

Product Specifications

Product Name Alternative

ELF1, E74-like factor 1 (ets domain transcription factor), OTTHUMP00000018310, ets-related transcription factor Elf-1

UniProt

P32519

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-AMKQNELLEPNSF

Target

ELF1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Human Lymph Node and Tonsil and in Mouse Thymus lysates (calculated MW of 64.9kDa according to Human NP_001138825.1 and 62.3kDa according to Mouse NP_001273341.1) . Recommended concentration: 0.1-0.3μg/mL. Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 67.5kDa according to NP_758961.1) . Recommended concentration: 0.03-0.1μg/mL.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 2.5μg/mL.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_001138825.1, NP_758961.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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