What is the catalog number for FH Antibody?

The catalog number for FH Antibody is orb1247472.

What sizes are available for FH Antibody?

FH Antibody is available in the following size(s): 0.1 mg.

Is FH Antibody in stock?

FH Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

FH Antibody

Product Specifications

Product Name Alternative

Fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata

UniProt

P07954

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-HPNDHVNKSQSSND

Target

FH

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2) . The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03μg/mL.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75μg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2μg/mL

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

EIA, ELISA, IHC, WB

NCBI Accession Number

NP_000134.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.