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TRIM55 Antibody

TRIM55 Antibody

Product Specifications

Product Name Alternative

TRIM55, RNF29, MURF-2, ring finger protein 29, for muscle specific ring finger 2, tripartite motif-containing 55, muscle specific ring finger 2

UniProt

Q9BYV6

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-HIFSFSWLNSLNE

Target

TRIM55

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 58kDa band observed in lysates of cell line HeLa (calculated MW of 60.5kDa according to NP_908973.1) . Recommended concentration: 1-3μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig, Rat and Mouse Heart, and Pig and Mouse Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 10μg/mL.Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, FC, IF, IHC, WB

NCBI Accession Number

NP_908973.1, NP_908974.1, NP_908975.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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