ROBO1 Antibody
ROBO1 Antibody
Product Specifications
Product Name Alternative
ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila), HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
UniProt
Q9Y6N7
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-GDVDLSNKINEMK
Target
ROBO1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:An anonymous source observed an approx 250kDa band in Human Endothelial cell lysates at 1μg/mL antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 2μg/mL.Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_598334.1, NP_002932.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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