SMAD9 Antibody
SMAD9 Antibody
Product Specifications
Product Name Alternative
SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila) ] , HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B , MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, MAD, mothers against decapentaplegic homolog 9 (Drosophila), Mothers against decapentaplegic, drosophila, homolog of, 9
UniProt
O15198
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-HSEPLMPHNATYPD
Target
SMAD9
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in Human Skin lysates (calculated MW of 48.6kDa according to NP_005896.1) . Recommended concentration: 0.3-1.0 μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SMAD9 captured by a rabbit antibody. Recommended reporter concentration: 1-2μg/mL
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
EIA, ELISA, IHC, WB
NCBI Accession Number
NP_005896.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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