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SMAD9 Antibody

SMAD9 Antibody

Product Specifications

Product Name Alternative

SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila) ] , HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B , MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, MAD, mothers against decapentaplegic homolog 9 (Drosophila), Mothers against decapentaplegic, drosophila, homolog of, 9

UniProt

O15198

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-HSEPLMPHNATYPD

Target

SMAD9

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in Human Skin lysates (calculated MW of 48.6kDa according to NP_005896.1) . Recommended concentration: 0.3-1.0 μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SMAD9 captured by a rabbit antibody. Recommended reporter concentration: 1-2μg/mL

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

EIA, ELISA, IHC, WB

NCBI Accession Number

NP_005896.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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