CLPB Antibody
CLPB Antibody
Product Specifications
Product Name Alternative
CLPB, ClpB caseinolytic peptidase B homolog (E. coli), FLJ13152, HSP78, SKD3, suppressor of potassium transport defect 3
UniProt
Q9H078
Immunogen
The immunogen for this antibody is: C-DKDSKTRRLDIRAP
Target
CLPB
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 50kDa and 48kDa in lysates of HeLa and HepG2 after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.7kDa according to NP_110440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Human
Tested Applications
ELISA
NCBI Accession Number
NP_110440.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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