ABCA2 Antibody
ABCA2 Antibody
Product Specifications
Product Name Alternative
ABCA2, ATP-binding cassette, sub-family A (ABC1), member 2, ABC2, MGC129761, ATP-binding cassette, sub-family A, member 2, OTTHUMP00000064733
UniProt
Q9BZC7
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-KKQSDNLEQQETEP
Target
ABCA2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation, Metabolism Research, Neuroscience, Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 270kDa according to NP_001597.2 and of 273kDa according to .NP_997698.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 3.75μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_001597.2, NP_997698.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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