NR1I2 Antibody
NR1I2 Antibody
Product Specifications
Product Name Alternative
PXR, NR1I2, nuclear receptor subfamily 1, group I, member 2, BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, SAR, SXR, orphan nuclear receptor, pregnane X receptor, steroid and xenobiotic receptor
UniProt
O75469
Reactivity
Human
Immunogen
The immunogen for this antibody is: EQFAITLKSYIECNR
Target
NR1I2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 48kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8kDa according to NP_003880.3) . Recommended concentration: 1-3μg/mL. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75μg/mL.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_003880.3, NP_148934.1, NP_071285.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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