IRF5 Antibody

Product Specifications

Product Name Alternative

IRF5, interferon regulatory factor 5, SLEB10

UniProt

Q13568

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-QGPWPMHPAGMQ

Target

IRF5

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Infectious Disease & Virology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Spleen and Peripheral Blood Lymphocytes lysates.The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.Immunohistochemistry:This antibody showed nuclear staining in Human Cervix, as confirmed by customer (data not shown) . This data is from a previous batch, not on sale.Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.This data is from a previous batch, not on sale.

Prediction Reactivity

Bovine, Mouse, Rat

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001092097.1, NP_116032.1, NP_002191.1, NP_001092099.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.