ATG5 Antibody
ATG5 Antibody
Product Specifications
Product Name Alternative
ATG5, ATG5 autophagy related 5 homolog (S. cerevisiae), APG5, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae) -like, APG5 autophagy 5-like, ATG5 autophagy related 5-like, OTTHUMP00000040507, apoptosis specific protein
UniProt
Q9H1Y0
Immunogen
The immunogen for this antibody is: C-SQVINEMQKKDHKQ
Target
ATG5
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx. 38 kDa band in NIH3T3 lysates after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4 kDa according to NP_004840.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Bovine, Equine, Human, Mouse
Tested Applications
ELISA
NCBI Accession Number
NP_004840.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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